ISG15 ELISA Kit for Bovine(Bos taurus) | EIAab.comISG15 ELISA Kit for Bovine(Bos taurus) | EIAab.com

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ISG15 (Gene name),

Ubiquitin-like protein ISG15 (Protein name ), ISG15_BOVIN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

ISG15(G1P2;ISG17;UCRP);

Protein name:

Ubiquitin-like protein ISG15;

Alternative:

Ubiquitin cross-reactive protein(BoUCRP);Interferon-stimulated gene product 17;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme).

Function:

In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.

Subcellular Location:

Cytoplasm Secreted UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.

Protein Attributes:

Sequence length:

154

Sequence:

50:

MGGDLTVKML | GGQEILVPLR | DSMTVSELKQ | FIAQKINVPA | FQQRLAHLDS |

100:

REVLQEGVPL | VLQGLRAGST | VLLVVQNCIS | ILVRNDKGRS | SPYEVQLKQT |

150:

VAELKQQVCQ | KERVQADQFW | LSFEGRPMDD | EHPLEEYGLM | KGCTVFMNLR |

154:

LRGG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

STRING:

9913.ENSBTAP00000019573

String:

KEGG:

UniGene:

Pfam:

Uniprot:

FOR

ELISA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

E14926h

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96T

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ELISA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

E14926b

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96T

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ELISA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

E14926m

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96T

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CLIA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

U14926m

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Packing:

96T

CLIA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

U14926h

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CLIA Kit for Bovine Ubiquitin-like protein ISG15

Cat.:

U14926b

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96T

Polyclonal Antibody for Bovine Ubiquitin-like protein ISG15

Polyclonal Antibody for Bovine Ubiquitin-like protein ISG15

Polyclonal Antibody for Bovine Ubiquitin-like protein ISG15

Monoclonal Antibody for Bovine Ubiquitin-like protein ISG15

Monoclonal Antibody for Bovine Ubiquitin-like protein ISG15

Monoclonal Antibody for Bovine Ubiquitin-like protein ISG15

Protein for Bovine Ubiquitin-like protein ISG15

Protein for Bovine Ubiquitin-like protein ISG15

Protein for Bovine Ubiquitin-like protein ISG15

R&D Technical Data

s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of ISG15 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	96-107
Plasma	103	94-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ISG15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-108%	98-111%	100-106%	99-109%
EDTA plasma(n=5)	95-111%	97-108%	98-108%	100-107%
heparin plasma(n=5)	98-106%	94-112%	96-109%	98-108%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Complementary deoxyribonucleic acid sequence encoding bovine ubiquitin cross-reactive protein: a comparison with ubiquitin and a 15-kDa ubiquitin homolog."
Austin K.J. , Pru J.K. , Hansen T.R.
Endocrine5:191-197(1996)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Endometrium.
2.

"Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription."
Perry D.J. , Austin K.J. , Hansen T.R.
Mol. Endocrinol.13:1197-1206(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];TISSUE SPECIFICITY
3.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2005-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Crossbred X Angus.
tissue: Ileum.
4.

"Ubiquitin cross-reactive protein is released by the bovine uterus in response to interferon during early pregnancy."
Austin K.J. , Ward S.K. , Teixeira M.G. , Dean V.C. , Moore D.W. , Hansen T.R.
Biol. Reprod.54:600-606(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION
5.

"Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium."
Hansen T.R. , Austin K.J. , Johnson G.A.
Endocrinology138:5079-5082(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
6.

"Pregnancy and interferon-tau induce conjugation of bovine ubiquitin cross-reactive protein to cytosolic uterine proteins."
Johnson G.A. , Austin K.J. , Van Kirk E.A. , Hansen T.R.
Biol. Reprod.58:898-904(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION

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